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A PDB file is DragDropped onto theĪpplication. User-friendly and usually fast (seconds-minutes for Module for PDB complexes and crystal contacts Protein/Protein and rather accurate protein/RNA binding sites predictions module PDB surface solvation (by water) and Coulomb Maker and editor of multiple sequence alignments PDB coordinates to yield insights that could Many of the features do not use multiple sequence Protein-DNA interface propensity, and conservations Physical covariation, protein-protein interface, Hydrophobicity conservation, conformational SEQMOL is a PDB structure analysis suite.īe used to align multiple protein and DNAĪttributes of multiple sequence alignments Moreover, it can be applied for the screening of FEN1 inhibitors and the monitoring of FEN1 activity in human cells, holding great potential in drug discovery and clinical diagnosis.Software for Deep Analysis of PDB Structures and Mechanism Discovery This method exhibits good specificity and high sensitivity with a limit of detection (LOD) of 1.75 × 10 −6 U μL −1. The ssRNA can hybridize with a molecular beacon to form an RNA/DNA heteroduplex that can be selectively digested by DSN to generate an enhanced fluorescence signal. Upon the addition of T7 RNA polymerase, an efficient T7 transcription amplification reaction is initiated to produce abundant single-stranded RNAs (ssRNAs). The ssDNA can hybridize with the T7 promoter-bearing template probe to trigger the extension with the aid of Klenow fragment (KF) DNA polymerase. In the presence of FEN1, the flapped dumbbell probe is cleaved to generate a free 5′ flap single-stranded DNA (ssDNA) with the 3′-OH terminus. Herein, we develop a target-activated T7 transcription circuit-mediated multiple cycling signal amplification platform for monitoring FEN1 activity in cancer cells. The structure-specific endonuclease flap endonuclease 1 (FEN1) is an essential functional protein in DNA replication and genome stability, and it has been identified as a promising biomarker and drug target for multiple cancers.